The present invention relates to a method of freeze drying, in particular to the freeze drying of cell based biological material and subsequent reconstitution. The biological material may be from any source such as microbial cells, protozoal cells, animal cells or plant cells. In particular the invention relates to freeze drying mammalian cells, such as blood cells, nucleated cells and bacterial cells for therapeutic use and use in food products. The invention further provides a method of reconstitution of the freeze dried cells resulting in better viability and functionality of the cells after reconstitution.

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