Comparative Analysis Of Bone Marrow and Wharton’s Jelly Mesenchymal Stem/Stromal Cells

Bone narrow (BM)- derived mesenchymal stem/stromal cells (MSCs) represent the most extensively studied population of adult MSCs and are considered as the gold-standard for MSC-based clinical applications. Yet, it is now becoming increasingly clear that BM may not represent the most suitable source for MSC collection. Indeed, Umbilical cord (UC) has emerged as a more abundant and easily attainable source of MSCs and several reports have shown that MSCs can be efficiently isolated from the connective tissue that surrounds UC vessels, namely the Wharton’s jelly (WJ). According to the existing literature, WJ-MSCs display typical MSC characteristics, however a head-to-head comparison with BM-MSCs is still lacking.

Compartive Analysis MSC product refs
By |2018-07-13T18:17:27+00:00July 13th, 2018|Bone Marrow, Mesenchymal Stem Cells, Stem Cells, Wharton’s Jelly|Comments Off on Comparative Analysis Of Bone Marrow and Wharton’s Jelly Mesenchymal Stem/Stromal Cells

Different populations and sources of human mesenchymal stem cells (MSC): A comparison of adult and neonatal tissue-derived MSC

The mesenchymal stroma harbors an important population of cells that possess stem cell-like characteristics including self renewal and differentiation capacities and can be derived from a variety of different sources. These multipotent mesenchymal stem cells (MSC) can be found in nearly all tissues and are mostly located in perivascular niches. MSC have migratory abilities and can secrete protective factors and act as a primary matrix for tissue regeneration during inflammation, tissue injuries and certain cancers. These functions underlie the important physiological roles of MSC and underscore a significant potential for the clinical use of distinct populations from the various tissues. MSC derived from different adult (adipose tissue, peripheral blood, bone marrow) and neonatal tissues (particular parts of the placenta and umbilical cord) are therefore compared in this mini-review with respect to their cell biological properties, surface marker expression and proliferative capacities. In addition, several MSC functions including in vitro and in vivo differentiation capacities within a variety of lineages and immune-modulatory properties are highlighted. Differences in the extracellular milieu such as the presence of interacting neighbouring cell populations, exposure to proteases or a hypoxic microenvironment contribute to functional developments within MSC populations originating from different tissues, and intracellular conditions such as the expression levels of certain micro RNAs can additionally balance MSC function and fate.

Comparison of Adult and Neonatal tissue MSC.hass2011
By |2018-07-13T16:45:45+00:00July 13th, 2018|Amniotic Allograft, Mesenchymal Stem Cells, Stem Cells, Umbilical Cord, Wharton’s Jelly|Comments Off on Different populations and sources of human mesenchymal stem cells (MSC): A comparison of adult and neonatal tissue-derived MSC

Collagen and glycosaminoglycans of Wharton’s jelly and their alterations in EPH-gestosiss

Some prenatal pathological processes may be caused by biochemical and morphological alterations in the umbilical cord (UC).EPH-gestosis is the most common pregnancy-associated pathological process. For these reasons the role of collagen and glycosaminoglycans (GAGS) of UC in pathobiochemistry of this syndrome seems be important. We studied histology of extracellular matrix components, quantity, solubility and molecular polymorphism of collagen, proportional relationships between various types of collagen, the amounts of GAGS and proportional relationships between them in Wharton’s jelly of control newborns delivered by healthy mothers and those delivered by mothers with EPH-gestosis. We found that Wharton’s jelly is abundant in collagen and GAGS. This collagen is very insoluble and resistant to the action of depolymerizing agents (4% EDTA-Na,, pepsin). Types I, III and V collagens were isolated and quantified. Hyaluronic acid constitutes about 70%, whereas sulphated GAGS constitute about 30% of total GAGS. EPH-gestosis is accompanied by significant increase in sulphated GAGS: hyaluronic acid ratio. The EPHgestosis- associated alterations in Wharton’s jelly correspond to ‘premature ageing’ of this tissue.

Collagen & Glyosaminoglycans of WJ.Bankowski1996
By |2018-07-13T16:25:30+00:00July 13th, 2018|Wharton’s Jelly|Comments Off on Collagen and glycosaminoglycans of Wharton’s jelly and their alterations in EPH-gestosiss

Antimicrobial Activity of Human Amniotic and Chorionic Membranes

The present study investigated the antimicrobial activity of human amniotic and chorionic membranes against some common bacterial and fungal pathogens. The findings clearly demonstrated the antimicrobial effect of both the amniotic and chorionic membranes against the tested bacterial and fungal pathogens at different dilutions by their maximum and minimum inhibitory zones. The maximum inhibition zone was measured in amniotic membrane compared to chorionic membrane in both the bacterial and fungal activity plates. While assessing the four different dilutions (5X105, 5X106, 5X107 and 5X108), the similar diameter of inhibition zone was observed in 1.5X105 and 1.5X106 dilutions. The study clearly confirmed the antimicrobial activity effect of both amniotic and chorionic membranes against several bacterial and fungal pathogens in which maximum activity was recorded by amniotic membrane.

Antimicrobial act Amniotic.Chorionic membrane.PARTHASARATHY2014
By |2018-07-13T16:18:46+00:00July 13th, 2018|Amniotic Allograft, Umbilical Cord, Wharton’s Jelly|Comments Off on Antimicrobial Activity of Human Amniotic and Chorionic Membranes