The Role of Biologic Therapy in Rotator Cuff Tears and Repairs

Rotator cuff tears are a growing problem within orthopedics as the incidence increases within an aging population, and repair of these tears has been estimated to save society approximately $ 3.4 Billion per year.

PRP-and-Allografts-in-Rotator-Cuff-Repairs-Feb-2018
By |2018-07-16T13:39:07+00:00July 16th, 2018|Adipose Tissue, Amniotic Allograft, Bone Marrow, Platelet Rich Plasma – PRP, Rotator Cuff|Comments Off on The Role of Biologic Therapy in Rotator Cuff Tears and Repairs

Freeze drying method

The present invention relates to a method of freeze drying, in particular to the freeze drying of cell based biological material and subsequent reconstitution. The biological material may be from any source such as microbial cells, protozoal cells, animal cells or plant cells. In particular the invention relates to freeze drying mammalian cells, such as blood cells, nucleated cells and bacterial cells for therapeutic use and use in food products. The invention further provides a method of reconstitution of the freeze dried cells resulting in better viability and functionality of the cells after reconstitution.

Freeze dry method
By |2018-07-13T18:20:00+00:00July 13th, 2018|Amniotic Allograft|Comments Off on Freeze drying method

Different populations and sources of human mesenchymal stem cells (MSC): A comparison of adult and neonatal tissue-derived MSC

The mesenchymal stroma harbors an important population of cells that possess stem cell-like characteristics including self renewal and differentiation capacities and can be derived from a variety of different sources. These multipotent mesenchymal stem cells (MSC) can be found in nearly all tissues and are mostly located in perivascular niches. MSC have migratory abilities and can secrete protective factors and act as a primary matrix for tissue regeneration during inflammation, tissue injuries and certain cancers. These functions underlie the important physiological roles of MSC and underscore a significant potential for the clinical use of distinct populations from the various tissues. MSC derived from different adult (adipose tissue, peripheral blood, bone marrow) and neonatal tissues (particular parts of the placenta and umbilical cord) are therefore compared in this mini-review with respect to their cell biological properties, surface marker expression and proliferative capacities. In addition, several MSC functions including in vitro and in vivo differentiation capacities within a variety of lineages and immune-modulatory properties are highlighted. Differences in the extracellular milieu such as the presence of interacting neighbouring cell populations, exposure to proteases or a hypoxic microenvironment contribute to functional developments within MSC populations originating from different tissues, and intracellular conditions such as the expression levels of certain micro RNAs can additionally balance MSC function and fate.

Comparison of Adult and Neonatal tissue MSC.hass2011
By |2018-07-13T16:45:45+00:00July 13th, 2018|Amniotic Allograft, Mesenchymal Stem Cells, Stem Cells, Umbilical Cord, Wharton’s Jelly|Comments Off on Different populations and sources of human mesenchymal stem cells (MSC): A comparison of adult and neonatal tissue-derived MSC

Antimicrobial Activity of Human Amniotic and Chorionic Membranes

The present study investigated the antimicrobial activity of human amniotic and chorionic membranes against some common bacterial and fungal pathogens. The findings clearly demonstrated the antimicrobial effect of both the amniotic and chorionic membranes against the tested bacterial and fungal pathogens at different dilutions by their maximum and minimum inhibitory zones. The maximum inhibition zone was measured in amniotic membrane compared to chorionic membrane in both the bacterial and fungal activity plates. While assessing the four different dilutions (5X105, 5X106, 5X107 and 5X108), the similar diameter of inhibition zone was observed in 1.5X105 and 1.5X106 dilutions. The study clearly confirmed the antimicrobial activity effect of both amniotic and chorionic membranes against several bacterial and fungal pathogens in which maximum activity was recorded by amniotic membrane.

Antimicrobial act Amniotic.Chorionic membrane.PARTHASARATHY2014
By |2018-07-13T16:18:46+00:00July 13th, 2018|Amniotic Allograft, Umbilical Cord, Wharton’s Jelly|Comments Off on Antimicrobial Activity of Human Amniotic and Chorionic Membranes